Latam Airlines has added Lima as its second destination in South America served directly from London.
The capital of Peru is being served five times a week from Heathrow, joining a daily service to Sao Paulo in Brazil.
The Lima route, announced in August, is being flown by 247-passenger Boeing 787 Dreamliner aircraft in a two-class configuration with departures on Mondays, Tuesdays, Wednesdays, Fridays and Sundays.
The 12-hour flight connects to Latam’s regional network in Peru and other countries in the region.
The carrier currently flies to 144 destinations in 24 countries, including the Falklands.
Regional commercial director Davide Ioppolo said: “Today we finally see the desire to connect the UK and Peru become a reality with the inauguration of this direct route between London and Lima.
“We are aware of the enthusiasm this announcement has generated in both countries and this gives us great expectations for the future.
“Our hub in Lima connects 19 destinations within Peru and more than 30 international destinations.
“If we add to this incredible network within South America the seven weekly frequencies between London and Sao Paulo, and the five frequencies of this route to Lima, Latam Airlines is positioned as the best option for anyone who wants to travel to South America, whether they are business or leisure travellers.”