Border Force officials are among more than 130,000 civil service workers who have voted to strike ahead of the May Day bank holiday weekend.
The latest one-day action by members of the PCS union on Friday, April 28 is alongside a month of walkouts starting on April 3 to increase the pressure on the government over pay, pensions and job security.
The latest action follows an all-out strike on March 15 to coincide with the Budget and walkouts earlier in the year.
The union represents hundreds of thousands who work in government departments as well as those at organisations such as Border Force, the Maritime and Coastguard Agency and Ofsted.
Members from 186 different employers were balloted over the latest strike action last week, the PCS union said.
General secretary Mark Serwotka said: “Our members are not backing down in this dispute. Ministers need to take notice that we’re escalating our action and they need to resolve the dispute by putting money on the table.
“We know our strikes have already caused serious disruption. The new strikes and another national day of action will pile the pressure on a government that refuses to listen.”
Advantage Travel Partnership chief executive Julia Lo Bue-Said said: “The Border Force strike action announced ahead of the May bank holiday is, once again, disappointing news for the travel industry. Fortunately, many of our travel agency partners are telling us that their customers are not currently being put off by the threat of strike action.
“For anyone that is travelling during this period, whether it’s for business or leisure, we would strongly advise that they prepare as much as possible for longer wait times, use e-gates where possible and check airport and port websites before travelling to see if any strikes are likely to affect their journeys.
“We do, however, expect that the majority of travellers will not experience delays as a result of contingency measures that are normally put in place to minimise disruption.”
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