Qatar Airways is to withdraw flights from Gatwick “for commercial reasons”.
The Doha-based airline will stop serving London’s second airport after May 31.
The move came after the Gulf carrier announced the addition of a second daily flight from Manchester from June 1.
The airline continues to operate from Heathrow.
Qatar Airways’ CEO Akbar Al Baker said: “We are extremely sad to be losing London Gatwick from our network after serving the route for the past seven years.
“But following a thorough review and analysis over the past few months, we have concluded that we could no longer sustain the route for commercial reasons.
“While we continue to grow our business, developing the network further, we do it prudently, cautiously and carefully.”