Flights from Cardiff airport to Glasgow are being withdrawn nine months after the route was re-instated.
CityJet, which operates three daily flights, said the route will be cut from October 25 but services to Paris and Edinburgh will continue.
It said it was “part of the process an airline goes through” while Cardiff airport said it remained a “key component” of CityJet’s strategy.
The airport was bought by the Welsh government in March last year for £52 million.
A year later, it reported a 9% increase in the number of passengers since the takeover.
Debra Barber, who took over as the airport’s managing director in August, said it was working closely with CityJet and its flights to Paris and Edinburgh “exceeded expectations” during the first year.
“Our priority is to return the business to its previous level of success and beyond with ongoing discussions with our existing and potential new airlines about enhancing the number of destinations on offer,” she told the BBC.