AIRIndia is pulling out of Manchester after deciding to redeploy aircraft on higher revenue routes.
The carrier flies the last of its four weekly flights to Delhi and Mumbai on April 30.
A Manchester Airport spokeswoman said Air India had indicated it wanted to use its Manchester-based Airbus A310s on more lucrative Middle East services.
The Manchester flights are routed through Rome before serving India, so the northwest airport will be left with just British Airways serving the Italian capital.
Up until the end of flights, Air India is offering a special ú79 Manchester-Rome return deal. Air India’s other UK service, to Heathrow, will operate as scheduled. Air India was unavailable for comment.