Interest in travel to Scotland from the rest of the UK has plummeted by almost a third.
And interest in travel from Scotland to other parts of the UK has also declined, but not at such a pace.
The findings from new research by hotel comparison website trivago.co.uk emerged as the Scottish referendum vote takes place today on whether the country should stay in the UK or become an independent nation.
Hotel searches to Scotland from England, Wales and Northern Ireland made between August 1 and September 15 dropped by 29% compared to the same period in 2013, the study shows.
Searches were for hotel stays for any date after September 28, in order to exclude searches made for the Ryder Cup golf tournament which takes place on September 23-28.
Even when hotel searches for the Ryder Cup were included, travel interest was still down by 19% year-on-year.
While UK interest to Scotland dropped, Scottish travel interest to the rest of the UK over the same period simultaneously declined by 19%.
Trivago spokeswoman Denise Bartlett said: “With the future of Scottish independence uncertain and many questioning how this will affect both potential visitors and the country’s tourism industry, it is interesting to note that Scotland has already seen a decline in travel interest from the rest of the UK.
“Despite the Ryder Cup, which was expected to boost Scottish tourism, searches for accommodation in the country have decreased in comparison to last year.
“Although this cannot be attributed wholly to the referendum, it is interesting to note that searches made by Scottish users to the rest of the UK have simultaneously decreased.
“With many in Scotland voting for independence, it appears likely that interest in visiting England, Wales and Northern Ireland has declined.”