Tui is urging holidaymakers to allow a minimum of three hours to check-in at Birmingham airport.
The new advice rises to four hours for those taking long-haul flights.
The airport blamed queues at security last weekend on passengers being unaware that restrictions limiting liquids to 100ml in hand luggage remain in place after a sudden government U-turn.
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This came despite Birmingham becoming one of the first UK airports to meet the government directive for new security equipment.
However, Tui said its reminder to passengers travelling from Birmingham was due to the airport getting a “face lift”, with a new £50 million security area being built.
The operator added in a travel alert on its website: “We’d like to remind you to arrive at the airport with sufficient time before your flight.
“At the moment, we’re recommending giving yourself three hours for flights to Europe and Africa, and four hours for long-haul flights.
“That way, you’ll have plenty of time to check in your luggage and make your way through the security queues. Arriving any earlier than this won’t mean you can check in or pass through security early.
“Together with Birmingham airport, we’re doing our best to make sure the facelift has the littlest effect as you pass through the airport. At the same time, we’re excited to be working behind the scenes to improve your future experience.”
Birmingham airport said: “Our new security area construction works are still on-going. This means the queuing area for security is mainly downstairs.
“Please note that all passengers arriving by train are required to head to the ground floor to join the queue for security.
“The airport has taken this opportunity to building for its future by building a new security search area that will be streamlined, speedier and simpler.”
Chief executive Nick Barton said: “The rules that we have been adhering to since March are now very clear in that only liquids, pastes and gels up to 100ml can be carried through in hand luggage.
“Since opening our new security area, and despite being one of the first UK airports to comply, we have been limited on the use of our multi-million-pound equipment due to an outstanding regulatory restriction meaning we had to limit liquids to 100ml. This rule has now been implemented nationwide.
“Despite the 100ml rule still being in place, we continually have non-compliant bags with liquids over the allowance which have led to inefficiencies of our equipment and resulted in extended queuing time for customers.
“It is now imperative that all customers comply with the nationwide rule to ensure a smoother and simpler transition through the airport. A non-compliant bag with liquids over 100ml can add up to 20 minutes to each passenger’s journey through security.
“All travelling customers should check their hand luggage to ensure it complies with this restriction.”
Similarly, Tui recommended the same check-in times at Manchester airport during a £1.3 billion revamp.